alt-r hdr donor oligos Search Results


crispr cas9 components  (Danaher Inc)
92
Danaher Inc crispr cas9 components
(a) Design of <t>CRISPR/Cas9</t> components. Five ssODN designs were compared. *: phosphorothioate bonds. (b) iPSCs were transfected with the ssODNs under different experimental conditions. Bulk populations were screened by MiSeq™. Populations with the highest rates of HDR were selected for colony picking, which were then screened using MiSeq™. Individual colonies showing accurate repair were expanded as isogenic lines.
Crispr Cas9 Components, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alt-r+hdr+donor+oligos/Alt-R+HDR+Donor+Oligos/bio_rxiv__2021__11__12__467071-67-1-6
Average 92 stars, based on 1 article reviews
crispr cas9 components - by Bioz Stars, 2026-10
92/100 stars
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(a) Design of CRISPR/Cas9 components. Five ssODN designs were compared. *: phosphorothioate bonds. (b) iPSCs were transfected with the ssODNs under different experimental conditions. Bulk populations were screened by MiSeq™. Populations with the highest rates of HDR were selected for colony picking, which were then screened using MiSeq™. Individual colonies showing accurate repair were expanded as isogenic lines.

Journal: bioRxiv

Article Title: An efficient and adaptable workflow for editing disease-relevant single nucleotide variants using CRISPR/Cas9

doi: 10.1101/2021.11.12.467071

Figure Lengend Snippet: (a) Design of CRISPR/Cas9 components. Five ssODN designs were compared. *: phosphorothioate bonds. (b) iPSCs were transfected with the ssODNs under different experimental conditions. Bulk populations were screened by MiSeq™. Populations with the highest rates of HDR were selected for colony picking, which were then screened using MiSeq™. Individual colonies showing accurate repair were expanded as isogenic lines.

Article Snippet: All CRISPR/Cas9 components were purchased through IDT. ssODNs were ordered as Alt-R™ HDR Donor Oligos.

Techniques: CRISPR, Transfection

(a) Design of CRISPR/Cas9 components targeting the G177D SNV in TBXT . The silent mutation introduced in the ssODN is shown in green. (b) After transfection, U-CH1 bulk populations were screened using digital droplet PCR (ddPCR). Populations showing HDR (2 to 10%) were single cell sorted into 96 well plates using FACS and were expanded into clonal cell lines. DNA was extracted from expanded clonal cell populations by establishing a “mirror plate”. Extracted DNA was used directly for genotyping by TaqMan™ qPCR or ddPCR and confirmed by Sanger sequencing. (c) Representative transmitted light microscopy picture of transfected U-CH1 showing satisfactory viability and morphology. (d) Dot plots showing the gating strategy for sorting U-CH1 cells based on TOPRO3 and ATTO-550 fluorescence by FACS.

Journal: bioRxiv

Article Title: An efficient and adaptable workflow for editing disease-relevant single nucleotide variants using CRISPR/Cas9

doi: 10.1101/2021.11.12.467071

Figure Lengend Snippet: (a) Design of CRISPR/Cas9 components targeting the G177D SNV in TBXT . The silent mutation introduced in the ssODN is shown in green. (b) After transfection, U-CH1 bulk populations were screened using digital droplet PCR (ddPCR). Populations showing HDR (2 to 10%) were single cell sorted into 96 well plates using FACS and were expanded into clonal cell lines. DNA was extracted from expanded clonal cell populations by establishing a “mirror plate”. Extracted DNA was used directly for genotyping by TaqMan™ qPCR or ddPCR and confirmed by Sanger sequencing. (c) Representative transmitted light microscopy picture of transfected U-CH1 showing satisfactory viability and morphology. (d) Dot plots showing the gating strategy for sorting U-CH1 cells based on TOPRO3 and ATTO-550 fluorescence by FACS.

Article Snippet: All CRISPR/Cas9 components were purchased through IDT. ssODNs were ordered as Alt-R™ HDR Donor Oligos.

Techniques: CRISPR, Mutagenesis, Transfection, Sequencing, Light Microscopy, Fluorescence

Proposed comprehensive workflow for editing stem cells or immortalised cell lines using CRISPR/Cas9.

Journal: bioRxiv

Article Title: An efficient and adaptable workflow for editing disease-relevant single nucleotide variants using CRISPR/Cas9

doi: 10.1101/2021.11.12.467071

Figure Lengend Snippet: Proposed comprehensive workflow for editing stem cells or immortalised cell lines using CRISPR/Cas9.

Article Snippet: All CRISPR/Cas9 components were purchased through IDT. ssODNs were ordered as Alt-R™ HDR Donor Oligos.

Techniques: CRISPR